For the isolation of Enterococcus faecalis
Applications
- Detection / Isolation
- ENTEROCOCCUS
Packaging
Suspend 56.1 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 10 minutes. Cool to 45-50ºC and aseptically add two vials of 1% TTC supplement (Cat. 6030), previously reconstituted in 5 ml of sterile distilled water. Homogenize gently and dispense into sterile containers.
Supplement: 100 mg of TTC in 10 ml of distilled water
Formula
- Maltose 20
- Proteose Peptone Nº3 10
- Yeast Extract 10
- Sodium Glycerophosphate 10
- Sodium Chloride 5
- Lactose 1
- Sodium Azide 0.4
- Bromocresol Purple 0.015
