Selective supplement for Brucella isolation
Packaging
Aseptically reconstitute 1 vial with 10 ml of 1:1 solution of methanol / sterile distilled water.Incubate at 37 °C for 10 -15 minutes.Mix until completely dissolved and aseptically add to 500 ml of BRUCELLA AGAR BASE(Cat.1012)or COLUMBIA AGAR BASE (Cat.1104),autoclaved,cooled to 50 °C and,if desired,with both 5 -10%inactivated horse serum and 1 -5%sterile dextrose solution added.Mix
well and distribute into sterile containers.
Formula
- Bacitracin 12500IU
- Cycloheximide 50mg
- Nalidixic Acid 2.5mg
- Nystatin 50000IU
- Polymyxin B 2500IU
- Vancomycin 10mg
