For the cultivation of anaerobic microorganisms from contaminated specimens
Applications
- Isolation / Cultivation
- ANAEROBIC BACTERIA
Packaging
Suspend 41.9 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121°C for 15 minutes. Cool to 45-50°C and, if desired, add 5% sterile defibrinated blood, homogenize gently and pour into Petri dishes. Be careful to avoid bubble formation when adding the blood.
Formula
- Trypticasein Soy Broth 10
- Peptone Mixture 5
- Dextrose 5
- Yeast Extract 5
- Tris (Hydroxymethyl Aminomethane) 3
- Hemin 0.01
- L-Cysteine 0.4
- Bacteriological Agar 13.5
